Beta-secretase 1 (BACE1) also known as beta-site APP cleaving enzyme 1 (beta-site amyloid precursor protein cleaving enzyme 1), memapsin-2 (membrane-associated aspartic protease 2), and aspartyl protease 2 (ASP2) is an enzyme that in humans is encoded by the BACE1 gene.  β-Secretase is an aspartic-acid protease important in the formation of myelin sheaths in peripheral nerve cells.

Faghihi, M. A et al., 2008 identified a conserved noncoding antisense transcript for β-secretase-1 (BACE1), a crucial enzyme in Alzheimer’s disease pathophysiology. The BACE1-antisense transcript (BACE1-AS) regulates BACE1 mRNA and subsequently BACE1 protein expression in vitro and in vivo. In order to find the regulatory effects of BACE1-AS the group performed the experiments on SH-SY5Y cell lines and transacted the cell lines by small interfering RNAs (siRNAs) targeting on the non-overlapping regions of BACE1-AS transcript and the observed surprising results. Along with the knockdown of the BACE1-AS, BACE1 mRNA were also knocked down confirming the regulatory effects on the BACE1-AS on BACE1 gene. (see figure)

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They repeated the similar experiments on two other cell lines and each time with three different siRNAs, all of them gave the similar outcome proving the concordant regulation of BACE1-AS on BACE1 gene.

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